recombinant mouse eotaxin (R&D Systems)
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recombinant mouse eotaxin
Recombinant Mouse Eotaxin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+eotaxin/Recombinant+Mouse+CCL11%2FEotaxin+Protein/bio_rxiv__64898__2026__02__13__705770-286-24-36
Average 94 stars, based on 17 article reviews
Recombinant Mouse Eotaxin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+eotaxin/Recombinant+Mouse+CCL11%2FEotaxin+Protein/bio_rxiv__64898__2026__02__13__705770-286-24-36
Average 94 stars, based on 17 article reviews
recombinant mouse eotaxin - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Microbiome depletion rejuvenates the aging brain"
Article Title: Microbiome depletion rejuvenates the aging brain
Journal: bioRxiv
doi: 10.64898/2026.02.13.705770
Figure Legend Snippet: a, Representative immunofluorescence images of Iba1+ (magenta) and CD68+ (green) microglia in the cortex of aged mice treated with water or antibiotics (Abx); quantification shows a significant reduction in both Iba1 and CD68 immunoreactivity following microbiome depletion. Yellow arrows indicate double positive cells (n = 5/group). Scale bar, 50 μm. b, Schematic of the experimental design: aged mice were treated with Abx or water for 30 days, after which plasma and cortical lysates were collected for cytokine profiling using a proteome array. c, Heatmaps showing fold change in cytokine and chemokine levels in plasma (left) and cortical lysates (right) relative to water controls. Multiple inflammatory mediators were downregulated upon Abx treatment; Eotaxin-1 (CCL11), highlighted in red, was significantly reduced in both compartments. d, Violin plots showing fold change in Eotaxin-1 abundance in plasma (top) and brain (bottom), confirming a significant reduction in circulating and central levels following microbiome depletion (plasma, n = 6/group; brain, n = 3/group). e, Schematic of rescue experiment: aged mice received antibiotics (Abx) to deplete the microbiome, in combination with either vehicle or recombinant Eotaxin-1 (n = 5/group). f-h, Quantification of blood vessel density (g), BrdU+ cells in the dentate gyrus (h), and CD68+ area in the cortex (i) (n = 5/group). All data represented as mean ± s.e.m.; * P < 0.05, ** P < 0.01, **** P < 0.0001, Student’s t -test (a,d); ANOVA with Tukey’s multiple-comparisons post hoc test (f-h).
Techniques Used: Immunofluorescence, Clinical Proteomics, Recombinant
Figure Legend Snippet: a, Schematic of the experimental design: aged mice were treated with either control IgG or an anti–eotaxin-1 neutralizing antibody for 30 days (Control, n = 3, Eotaxin Ab, n = 5). b, Representative CD31 immunostaining and quantification showing increased blood vessel density in the cortex of Eotaxin-1–inhibited mice. c, BrdU labeling (green, arrows) of proliferating cells in the DG and quantification of BrdU+ cells reveals enhanced adult neurogenesis following Eotaxin-1 inhibition. d, Immunofluorescence for Iba1 (magenta) and CD68 (green) shows reduced microglial activation in Eotaxin-1–treated mice. Yellow arrows indicate double positive cells. e, Quantification of myelinating oligodendrocyte precursor cells (Pdgfra+, green) in the corpus callosum (highlighted in white) indicates increased myelination upon Eotaxin-1 blockade. Scale bars: 100 μm (b), 50 μm (c,d), 500 μm (e). f, Schematic of behavioral test design: aged mice treated with Rat IgG or Eotaxin-1 antibody were tested for locomotor function and anxiety using the Open Field (OF) test, and their memory was assessed using the novel object recognition test (NOR) test. g, Time (sec) and distance spent (m) in the center or periphery of the arena during the OF test. h, Discrimination index, exploration time (sec) and total distance travelled (m) in the NOR test (n = 7/control; 10/Eotaxin-1 antibody). All data represented as mean ± s.e.m.; * P < 0.05, ** P < 0.01, Student’s t -test.
Techniques Used: Control, Immunostaining, Labeling, Inhibition, Immunofluorescence, Activation Assay
Figure Legend Snippet: a, Flow cytometry analysis of peripheral blood from aged mice showing the frequency of major immune cell populations, including eosinophils, following Abx treatment. b, Representative images of CD170+ eosinophils (green) in the intestinal epithelium of young, aged control, and aged Abx-treated mice. c, Quantification of CD170+ eosinophils normalized to intestinal villus area in young, aged control, and aged Abx-treated mice (n = 4/group). d, Quantification of CD170+ eosinophils in the intestinal villi of aged mice treated with water + PBS or Abx + Eotaxin-1 (n = 3 control, n = 4 Abx + Eotaxin). White arrows indicate CD170 positive cells. Scale bar 100 µm. All data represented as mean ± s.e.m.; * P < 0.05, ** P < 0.01. ANOVA with Tukey’s multiple-comparisons post hoc test (c), Student’s t -test (d).
Techniques Used: Flow Cytometry, Control
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